Engineering an FeFe-hydrogenase: do accessory clusters influence O2 resistance and catalytic bias?

G Caserta, C Papini, A Adamska-Venkatesh, L Pecqueur, C Sommer, E Reijerse, W Lubitz, C Gauquelin, I Meynial-Salles, D Pramanik, V Artero, M Atta, M del Barrio, B Faivre, V Fourmond, C Léger and M Fontecave, J. Am. Chem. Soc., DOI: 10.1021/jacs.8b01689 Publication Date (Web): March 29, 2018 Continue reading

Electrochemical Investigations of Hydrogenases and Other Enzymes That Produce and Use Solar Fuels

Melisa del BarrioMatteo Sensi Christophe OrainCarole BaffertSébastien DementinVincent Fourmond , and Christophe Léger. Acc. Chem. Res.201851 (3), pp 769–777 DOI: 10.1021/acs.accounts.7b00622 Publication Date (Web): March 8, 2018


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Reactivity of the excited states of the H-cluster of FeFe hydrogenases

M Sensi, C Baffert, C Greco, G Caserta, C Gauquelin, L Saujet, M Fontecave, S Roy, V Artero, P Soucaille, I Meynial-Salles, H Bottin, L de Gioia, V Fourmond, Christophe Leger and L Bertini, “Reactivity of the excited states of the H-cluster of FeFe hydrogenases,” J. Am. Chem. Soc.138 13162-13618 (2016)


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Mechanism of O2 diffusion and reduction in [FeFe] hydrogenases

Adam Kubas, Christophe Orain, David De Sancho, Laure Saujet, Matteo Sensi, Charles Gauquelin, Isabelle Meynial-Salles, Philippe Soucaille, Hervé Bottin, Carole Baffert, Vincent Fourmond, Robert B. Best, Jochen Blumberger* and Christophe Léger*, Nature Chemistry, 9, 88-95 (2017)DOI: 10.1038/NCHEM.2592

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Electrochemical measurements of the kinetics of inhibition of two FeFe hydrogenases by O2 demonstrate that the reaction is partly reversible

Christophe Orain, Laure Saujet, Charles Gauquelin, Philippe Soucaille, Isabelle Meynial-Salles, Carole Baffert, Vincent Fourmond, Hervé Bottin, Christophe Léger, J. Am. Chem. Soc137, 12580-12587 (2015) DOI: 10.1021/jacs.5b06934

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Combining experimental and theoretical methods to learn about the reactivity of gas-processing metalloenzymes (open access)

Claudio Greco, Vincent Fourmond, Carole Baffert, Po-Hung Wang, Sébastien Dementin, Patrick Bertrand, Maurizio Bruschi, Jochen Blumberger, Luca de Gioia, Christophe Léger. Energy Environ. Sci., 7, 3543-3573 (2014) DOI: 10.1039/C4EE01848F (open access)


Relating diffusion along the substrate tunnel and oxygen sensitivity in hydrogenase

P.-P. Liebgott, F. Leroux, B. Burlat, S. Dementin, C. Baffert, Th. Lautier, V. Fourmond, P. Ceccaldi, Ch. Cavazza, I. Meynial-Salles, Ph. Soucaille, J. Fontecilla-Camps, B. Guigliarelli, P. Bertrand, M. Rousset, Ch. Leger

Nat. Chem. Biol. 6(1) 63-70 (2010) doi:10.1038/nchembio.276

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