Photoinhibition of FeFe Hydrogenase

Matteo Sensi, Carole Baffert, Laura Fradale, Charles Gauquelin, Philippe Soucaille, Isabelle Meynial-Salles, Hervé Bottin, Luca de Gioia, Maurizio Bruschi, Vincent Fourmond, Christophe Léger, and Luca Bertini. ACS Catal., 2017, 7, pp 7378–7387 DOI: 10.1021/acscatal.7b02252

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New perspectives in hydrogenase direct electrochemistry

M Sensi, M del Barrio, C Baffert, V Fourmond, C Leger “New perspectives in hydrogenase direct electrochemistry”, Current Opinion in Electrochemistry (2017) doi:10.1016/j.coelec.2017.08.005

Roles of the F-domain in [FeFe] hydrogenase

Charles Gauquelin; Carole Baffert; Pierre Richaud; Emma Kamionka; Emilien  Etienne; David Guieysse; Laurence Girbal; Vincent Fourmond; Isabelle André; Bruno Guigliarelli; Christophe Léger; Philippe Soucaille; Isabelle Meynial-salles. BBA – Bioenergetics

Modelling the voltammetry of adsorbed enzymes and molecular catalysts

V Fourmond and C. Leger, Curr. Op. Electrochem (2017) doi:10.1016/j.coelec.2016.11.002

Reactivity of the excited states of the H-cluster of FeFe hydrogenases

M Sensi, C Baffert, C Greco, G Caserta, C Gauquelin, L Saujet, M Fontecave, S Roy, V Artero, P Soucaille, I Meynial-Salles, H Bottin, L de Gioia, V Fourmond, Christophe Leger and L Bertini, “Reactivity of the excited states of the H-cluster of FeFe hydrogenases,” J. Am. Chem. Soc.138 13162-13618 (2016) http://pubs.acs.org/doi/abs/10.1021/jacs.6b06603

Mechanism of O2 diffusion and reduction in [FeFe] hydrogenases

Adam Kubas, Christophe Orain, David De Sancho, Laure Saujet, Matteo Sensi, Charles Gauquelin, Isabelle Meynial-Salles, Philippe Soucaille, Hervé Bottin, Carole Baffert, Vincent Fourmond, Robert B. Best, Jochen Blumberger* and Christophe Léger*, Nature Chemistry, 9, 88-95 (2017), DOI: 10.1038/NCHEM.2592

Movie: MD “pulling” experiment showing O2 access to the H-cluster of FeFe hydrogenase

Electrochemical measurements of the kinetics of inhibition of two FeFe hydrogenases by O2 demonstrate that the reaction is partly reversible

Christophe Orain, Laure Saujet, Charles Gauquelin, Philippe Soucaille, Isabelle Meynial-Salles, Carole Baffert, Vincent Fourmond, Hervé Bottin, Christophe Léger, J. Am. Chem. Soc. 137, 12580-12587 (2015) DOI: 10.1021/jacs.5b06934

L’electrochimie, un outil pour étudier les mécanismes enzymatiques

Carole Baffert, Sébastien Dementin, Vincent Fourmond, Christophe Léger. L’actualité Chimique, 392, 9-15 (2015), http://www.lactualitechimique.org/spip.php?numero_article3224

Combining experimental and theoretical methods to learn about the reactivity of gas-processing metalloenzymes (open access)

Claudio Greco, Vincent Fourmond, Carole Baffert, Po-Hung Wang, Sébastien Dementin, Patrick Bertrand, Maurizio Bruschi, Jochen Blumberger, Luca de Gioia, Christophe Léger. Energy Environ. Sci., 7, 3543-3573 (2014) DOI: 10.1039/C4EE01848F (open access)

Movie: MD trajectory showing the flexibility of the protein around the H-cluster of FeFe hydrogenase

Based on the trajectory calculated for this work by Jochen Blumberger and Po-hung Wang.

The oxidative inactivation of FeFe hydrogenase reveals the flexibility of the H-cluster

Fourmond V, Greco C, Sybirna K, Baffert C, Wang PH, Ezanno P, Montefiori M, Bruschi M, Meynial-Salles I, Soucaille P, Blumberger J, Bottin H, De Gioia L, Léger C. Nature Chemistry 6(4):336-42 (2014) doi: 10.1038/nchem.1892.

Reductive inactivation of FeFe hydrogenase and implication for catalysis

V Hajj, C Baffert, K Sybirna, I Meynial-Salles, P Soucaille, H Bottin, V Fourmond, C Léger. Energy Environ. Sci., 2014,7, 715-719. DOI: 10.1039/C3EE42075B

 

Does the environment around the H-cluster allow coordination of the pendant amine to the catalytic iron center in FeFe hydrogenases? Answers from theory

Miyake T, Bruschi M, Cosentino U, Baffert C, Fourmond V, Léger C, Moro G, De Gioia L, Greco C. J Biol Inorg Chem. 2013 Aug;18(6):693-700. doi: 10.1007/s00775-013-1014-4.

The mechanism of inhibition by H2 of H2-evolution by hydrogenases

Fourmond V, Baffert C, Sybirna K, Dementin S, Abou-Hamdan A, Meynial-Salles I, Soucaille P, Bottin H, Léger C. Chem Commun (Camb). 2013 Aug 7;49(61):6840-2. doi: 10.1039/c3cc43297a. Epub 2013 Jun 24

Arginine 171 of Chlamydomonas reinhardtii FeFe hydrogenase HydA1 plays a crucial role in electron transfer to its catalytic center

Kateryna Sybirna, P Ezanno, C Baffert, C Leger, H Bottin. International Journal of Hydrogen Energy Volume 38, Issue 7, 7 March 2013, Pages 2998–3002

Steady-state catalytic wave-shapes for 2-electron reversible electrocatalysts and enzymes

Fourmond V, Baffert C, Sybirna K, Lautier T, Abou Hamdan A, Dementin S, Soucaille P, Meynial-Salles I, Bottin H, Léger C. J Am Chem Soc. 2013 ;135(10):3926-38. doi: 10.1021/ja311607s.

Structure et fonction des hydrogénases (fr)

Chapitre du livre L’énergie à découvert (CNRS ed. 2013)

Table des matières / Le chapitre

Covalent attachment of FeFe hydrogenases to carbon electrodes for direct electron transfer

Baffert C, Sybirna K, Ezanno P, Lautier T, Hajj V, Meynial-Salles I, Soucaille P, Bottin H, Léger C. Anal Chem. 2012 Sep 18;84(18):7999-8005. doi: 10.1021/ac301812s.